Preparation of micrometer-sized free-standing membranes

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Fabian Heinemann - , TUD Dresden University of Technology (Author)
  • Petra Schwille - , Chair of Biophysics (Author)

Abstract

Snap, crackle and pop! A simple method to obtain solvent-free, free-standing membranes suspended over apertures with 2.5 μm in diameter is described. The membranes are obtained by bursting of giant unilamellar vesicles (GUV) on a holey surface (see picture). Membrane integrity after bursting is fostered by enhancing the electrostatic interaction of the holey surface and the lipids.

Details

Original languageEnglish
Pages (from-to)2568-2571
Number of pages4
JournalChemPhysChem
Volume12
Issue number14
Publication statusPublished - 4 Oct 2011
Peer-reviewedYes

Keywords

Keywords

  • bilayers, fluorescence spectroscopy, lipids, membranes, vesicles