Topological photonics offers a versatile platform for designing advanced photonic materials and devices, such as one-way waveguides, on-chip optical isolators, and topologically protected lasers. Recently, particular interest has emerged in exploring topologically protected polariton lasing. However, current topological polariton lasers mainly rely on inorganic semiconductors, suffering from cryogenic temperature operation and limited applicability due to the small binding energies of Wannier–Mott excitons. Here, we realize room-temperature topological polariton lasers by exploiting tightly bound Frenkel excitons in organic semiconductor lattices. The Frenkel excitons strongly couple with microcavity photons to generate stable exciton polaritons. By modulation of the intra- and intercell coupling strengths of organic semiconductor lattices, room-temperature polariton lasing is achieved at topological edge states. The topological polariton laser exhibits a temperature-controlled wavelength-tunable laser output. This work demonstrates organic semiconductors as promising material systems for topological polariton devices.