Phenytoin inhibits expression of microtubule-associated protein 2 and influences cell-viability and neurite growth of cultured cerebellar granule cells

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • Gerd Kempermann - , University of Freiburg (Author)
  • Benedikt Volk - , University of Freiburg (Author)

Abstract

The objective of this study was to show whether an in vitro model for Phenytoin-related cytoskeletal impairment could be helpful to investigate cerebellar side effects of Phenytoin (DPH). DPH dose-and time-dependently resulted in decreasing numbers of vital cells. Cells formed only a rarefield intercellular neuritic network. This effect was already evident 24 h after plating. Western-blot analysis revealed that the expression of the dendritic marker microtubule-associated protein 2 (MAP2) was dramatically decreased in DPH-treated cultures.

Details

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalBrain research
Volume687
Issue number1-2
Publication statusPublished - 31 Jul 1995
Peer-reviewedYes
Externally publishedYes

External IDs

PubMed 7583305
ORCID /0000-0002-5304-4061/work/152544180

Keywords

ASJC Scopus subject areas

Keywords

  • Cell culture, Cerebellum, Cytoskeleton, MAP2, Mouse brain, Phenytoin