Peptide antibiotic sensing and detoxification modules of Bacillus subtilis
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Peptide antibiotics are produced by a wide range of microorganisms. Most of them target the cell envelope, often by inhibiting cell wall synthesis. One of the resistance mechanisms against antimicrobial peptides is a detoxification module consisting of a two-component system and an ABC transporter. Upon the detection of such a compound, the two-component system induces the expression of the ABC transporter, which in turn removes the antibiotic from its site of action, mediating the resistance of the cell. Three such peptide antibiotic-sensing and detoxification modules are present in Bacillus subtilis. Here we show that each of these modules responds to a number of peptides and confers resistance against them. BceRS-BceAB (BceRS-AB) responds to bacitracin, plectasin, mersacidin, and actagardine. YxdJK-LM is induced by a cationic antimicrobial peptide, LL-37. The PsdRS-AB (formerly YvcPQ-RS) system responds primarily to lipid II-binding lantibiotics such as nisin and gallidermin. We characterized the psdRS-AB operon and defined the regulatory sequences within the P(psdA) promoter. Mutation analysis demonstrated that P(psdA) expression is fully PsdR dependent. The features of both the P(bceA) and P(psdA) promoters make them promising candidates as novel whole-cell biosensors that can easily be adjusted for high-throughput screening.
Details
Original language | English |
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Pages (from-to) | 515-25 |
Number of pages | 11 |
Journal | Antimicrobial agents and chemotherapy |
Volume | 55 |
Issue number | 2 |
Publication status | Published - 20 Jan 2011 |
Peer-reviewed | Yes |
External IDs
PubMedCentral | PMC3028804 |
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Scopus | 78751685410 |
Keywords
Keywords
- ATP-Binding Cassette Transporters/genetics, Anti-Bacterial Agents/metabolism, Antimicrobial Cationic Peptides/metabolism, Bacillus subtilis/drug effects, Bacitracin/metabolism, Bacterial Proteins/genetics, Bacteriocins/metabolism, Biosensing Techniques, Drug Resistance, Bacterial, Gene Expression Regulation, Bacterial, Operon, Peptides/metabolism, Signal Transduction