A wide range of processes in the fields of medicine, biotechnology, process engineering, as well as chemical analyses require a specific detection and quantification of certain substances like heavy metals, antibiotics, or metabolic intermediates. Numerous recent studies have clearly demonstrated the ability of biological transducers to cope with such measurement tasks. This paper presents the development of an integrated Whole-Cell sensor, which is based on the detection of a dependent fluorescence. Thereby we investigate the potential of a compact and robust optical biosensor.