Arable fields and mesic meadows have been affected by intensifying agricultural management and nutrient input during the 20th century, but direct evidence for the long-term impact of intensification on plant nutrient contents remains scarce. Non-destructive novel spectroscopic methods can produce such data from herbarium specimens, making it possible to investigate how contents of leaf nutrient traits, especially nitrogen and phosphorus, changed over the last century, and what role habitat type and management practices play. We carried out a resurvey study of functional traits in arable field and mesic meadow communities. We used specimens from two German herbaria with a high coverage of their local floras: the herbaria Senckenberg Görlitz and Senckenberg Haussknecht in Jena. Following specimen information, the same plant species were resampled in the field in 2022 at the same locations. We employed near-infrared spectroscopy to predict leaf nitrogen, phosphorus and carbon content of herbarium and field specimens. Nutrient content changes over time were compared with public records of regional P and N fertilization. Overall, 1270 specimens of 76 species from both herbarium and field were studied, the oldest from the 19th century. Leaf nitrogen and the leaf nitrogen:phosphorus ratio increased significantly through time, while leaf phosphorus and carbon content decreased significantly over time. Arable field species showed a stronger response in leaf phosphorus content and leaf nitrogen:phosphorus ratio than mesic meadow species. The total amount of nitrogen or phosphorus fertilizer applied per year on a regional scale was found to be significantly correlated with the respective leaf nutrient content levels. Synthesis: Our study shows a long-term increase of leaf nitrogen in the studied habitats, paralleling increased chemical fertilizer applications in Germany. Our data indicate a shift from predominantly N-limited towards more P-limited growth conditions. The stronger response of species from arable fields compared to species from mesic meadows could indicate a faster adjustment to environmental pressures. This study thus also serves to showcase the potential of the combination of herbarium collections and NIR spectroscopy.