Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportContributedpeer-review

Contributors

Abstract

During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.

Details

Original languageEnglish
Title of host publicationAnimal Models of Diabetes
PublisherSpringer Science and Business Media, LLC
Pages159-179
Number of pages21
Volume2128
ISBN (electronic)978-1-0716-0385-7
ISBN (print)978-1-0716-0384-0
Publication statusPublished - Mar 2020
Peer-reviewedYes

Publication series

SeriesMethods In Molecular Biology

External IDs

PubMed 32180193
Scopus 85082021650

Keywords

Keywords

  • Brainbow, Heterogeneity, Lineage tracing, Quiescence, Single cell