Monitoring the maturation of the sarcomere network: a super-resolution microscopy-based approach

Research output: Contribution to journalResearch articleContributedpeer-review


  • Anna Skorska - , University of Rostock (Author)
  • Lisa Johann - , University of Rostock (Author)
  • Oleksandra Chabanovska - , University of Rostock (Author)
  • Praveen Vasudevan - , University of Rostock (Author)
  • Sophie Kussauer - , University of Rostock (Author)
  • Maximilian Hillemanns - , University of Rostock (Author)
  • Markus Wolfien - , Institute for Medical Informatics and Biometry, University of Rostock (Author)
  • Anika Jonitz-Heincke - , University of Rostock (Author)
  • Olaf Wolkenhauer - , University of Rostock, University of Stellenbosch (Author)
  • Rainer Bader - , University of Rostock (Author)
  • Hermann Lang - , University of Rostock (Author)
  • Robert David - , University of Rostock (Author)
  • Heiko Lemcke - , University of Rostock (Author)


The in vitro generation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC) is of great importance for cardiac disease modeling, drug-testing applications and for regenerative medicine. Despite the development of various cultivation strategies, a sufficiently high degree of maturation is still a decisive limiting factor for the successful application of these cardiac cells. The maturation process includes, among others, the proper formation of sarcomere structures, mediating the contraction of cardiomyocytes. To precisely monitor the maturation of the contractile machinery, we have established an imaging-based strategy that allows quantitative evaluation of important parameters, defining the quality of the sarcomere network. iPSC-derived cardiomyocytes were subjected to different culture conditions to improve sarcomere formation, including prolonged cultivation time and micro patterned surfaces. Fluorescent images of α-actinin were acquired using super-resolution microscopy. Subsequently, we determined cell morphology, sarcomere density, filament alignment, z-Disc thickness and sarcomere length of iPSC-derived cardiomyocytes. Cells from adult and neonatal heart tissue served as control. Our image analysis revealed a profound effect on sarcomere content and filament orientation when iPSC-derived cardiomyocytes were cultured on structured, line-shaped surfaces. Similarly, prolonged cultivation time had a beneficial effect on the structural maturation, leading to a more adult-like phenotype. Automatic evaluation of the sarcomere filaments by machine learning validated our data. Moreover, we successfully transferred this approach to skeletal muscle cells, showing an improved sarcomere formation cells over different differentiation periods. Overall, our image-based workflow can be used as a straight-forward tool to quantitatively estimate the structural maturation of contractile cells. As such, it can support the establishment of novel differentiation protocols to enhance sarcomere formation and maturity.


Original languageEnglish
Article number149
JournalCellular and Molecular Life Sciences
Issue number3
Publication statusPublished - Mar 2022

External IDs

PubMed 35199227
ORCID /0000-0002-1887-4772/work/143075285


Sustainable Development Goals


  • iPSC cardiomyocytes, Maturation, Sarcomere network, Super resolution microscopy