Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • K. V. Okuda - , University Hospital Carl Gustav Carus Dresden, Department of Child and Adolescent Psychiatry and Psychotherapy (Author)
  • N. Toepfner - , Department of Paediatrics, University Hospital Carl Gustav Carus Dresden (Author)
  • A. S. Alabi - , Centre de Recherches Médicales de Lambaréné (CERMEL), University of Tübingen (Author)
  • B. Arnold - , Department of Paediatrics, University Hospital Carl Gustav Carus Dresden (Author)
  • S. Bélard - , University of Tübingen, Charité – Universitätsmedizin Berlin (Author)
  • U. Falke - , University Hospital Carl Gustav Carus Dresden, Department of Child and Adolescent Psychiatry and Psychotherapy (Author)
  • L. Menschner - , Department of Paediatrics, University Hospital Carl Gustav Carus Dresden (Author)
  • S. Monecke - , Alere Technologies GmbH/InfectoGnostics Research Campus, Institute of Medical Microbiology and Virology (Author)
  • A. Ruppelt-Lorz - , Institute of Medical Microbiology and Virology (Author)
  • R. Berner - , Department of Paediatrics, University Hospital Carl Gustav Carus Dresden (Author)

Abstract

While there is an abundance of data on the epidemiology and molecular typing of Staphylococcus aureus, especially those carrying Panton–Valentine leucocidin (PVL) genes or mecA from Western Europe, Northern America and Australia, comparably few studies target African strains. In this study, we characterised genes associated with virulence and resistance, as well the phylogenetic background of S. aureus from healthy carriers and outpatients in Gabon. In total, 103 isolates from 96 study participants were characterised. Seventy-nine isolates originated from throat swabs and 24 isolates from skin lesions. Three isolates carried mecA, although only one, belonging to CC8-MRSA-IV [PVL+] ‘USA300’, was found to be phenotypically oxacillin-resistant; two CC88-MRSA-IV isolates appeared to be oxacillin-susceptible. PVL genes were common, with a total of 44 isolates (43 %) found to be PVL-positive. CC15-MSSA [PVL+] (n = 29) and CC152-MSSA [PVL+] (n = 9) were the predominant clones among the PVL-positive isolates. Among PVL-negative isolates, CC5-MSSA (n = 12), CC101-MSSA (n = 10) and CC15 (n = 9) were the most frequent. A hitherto undescribed multilocus sequence type of S. schweitzeri was detected twice in unrelated patients. The data emphasise a need for further studies on the role of PVL in African populations and the clinical significance of S. schweitzeri.

Details

Original languageEnglish
Pages (from-to)1963-1973
Number of pages11
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume35
Issue number12
Publication statusPublished - 1 Dec 2016
Peer-reviewedYes

External IDs

PubMed 27553495

Keywords

Sustainable Development Goals