Poly(ether amide) dendrimers with different molar masses were investigated using Size Exclusion Chromatography (SEC) with linear standard calibration and Matrix Assisted Laser Desorption/Ionization - Time-of-Flight - Mass Spectroscopy (MALDI-TOF-MS). The SEC behaviour of the dendrimers was investigated in different column systems, in pure and in mixed N,N-dimethylacetamide with water and LiCl. This behaviour was compared to linear poly(styrene) and poly(2-vinyl pyridine) standards. Kuhn-Mark-Houwink-Sakurada relationship yielded evidence about the molecular shapes of the polymers in solution and explained the different solution behaviour of the polymers. The universal calibration method was tested for its application to molar mass determination of poly(ether amide) dendrimers.