Minimal information for studies of extracellular vesicles (MISEV2023):From basic to advanced approaches

Research output: Contribution to journalResearch articleContributedpeer-review


  • Joshua A Welsh - , National Cancer Institute (Author)
  • Deborah C I Goberdhan - , University of Oxford (Author)
  • Lorraine O'Driscoll - , Trinity College Dublin (Author)
  • Edit I Buzas - , Semmelweis University (Author)
  • Cherie Blenkiron - , The University of Auckland (Author)
  • Benedetta Bussolati - , University of Turin (Author)
  • Houjian Cai - , University of Georgia (Author)
  • Dolores Di Vizio - , Cedars-Sinai Medical Center (Author)
  • Tom A P Driedonks - , University Medical Center (UMC) Utrecht (Author)
  • Uta Erdbrügger - , University of Virginia Health System (Author)
  • Juan M Falcon-Perez - , Center for Cooperative Research in Biosciences (Author)
  • Qing-Ling Fu - , Sun Yat-Sen University (Author)
  • Andrew F Hill - , Victoria University (Author)
  • Metka Lenassi - , University of Ljubljana (Author)
  • Sai Kiang Lim - , Agency for Science, Technology and Research, Singapore (Author)
  • Mỹ G Mahoney - , Thomas Jefferson University (Author)
  • Sujata Mohanty - , All India Institute of Medical Sciences, New Delhi (Author)
  • Andreas Möller - , Chinese University of Hong Kong (Author)
  • Rienk Nieuwland - , University of Amsterdam (Author)
  • Takahiro Ochiya - , Tokyo Medical University (Author)
  • Susmita Sahoo - , Icahn School of Medicine at Mount Sinai (Author)
  • Ana C Torrecilhas - , Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários (Author)
  • Lei Zheng - , Southern Medical University (Author)
  • Andries Zijlstra - , Vanderbilt University (Author)
  • Sarah Abuelreich - , Beckman Research Institute of the City of Hope (Author)
  • Reem Bagabas - , Beckman Research Institute of the City of Hope (Author)
  • Paolo Bergese - , University of Brescia (Author)
  • Esther M Bridges - , University of Oxford (Author)
  • Marco Brucale - , Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati (Author)
  • Dylan Burger - , Ottawa Hospital Research Institute (Author)
  • Randy P Carney - , Dominican University of California (Author)
  • Emanuele Cocucci - , Ohio State University (Author)
  • Federico Colombo - , Ohio State University (Author)
  • Rossella Crescitelli - , University of Gothenburg (Author)
  • Edveena Hanser - , University Hospital Basel (Author)
  • Adrian L Harris - , University of Oxford (Author)
  • Norman J Haughey - , Johns Hopkins Medicine (Author)
  • An Hendrix - , Ghent University (Author)
  • Alexander R Ivanov - , Northeastern University (Author)
  • Tijana Jovanovic-Talisman - , Beckman Research Institute of the City of Hope (Author)
  • Nicole A Kruh-Garcia - , Colorado State University (Author)
  • Vroniqa Ku'ulei-Lyn Faustino - , Beckman Research Institute of the City of Hope (Author)
  • Diego Kyburz - , University of Basel (Author)
  • Cecilia Lässer - , University of Gothenburg (Author)
  • Kathleen M Lennon - , Beckman Research Institute of the City of Hope (Author)
  • Jan Lötvall - , University of Gothenburg (Author)
  • Adam L Maddox - , Beckman Research Institute of the City of Hope (Author)
  • Elena S Martens-Uzunova - , Erasmus University Rotterdam (Author)
  • Rachel R Mizenko - , Dominican University of California (Author)
  • Lauren A Newman - , Flinders University (Author)
  • Corbeil D (Misev Consortium) - , Research Group Tissue Engineering, Tissue Engineering (Research Group) (Author)


Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.


Original languageEnglish
Article numbere12404
Pages (from-to)1-84
Number of pages84
JournalJournal of extracellular vesicles
Issue number2
Publication statusPublished - 7 Feb 2024

External IDs

ORCID /0000-0003-1181-3659/work/153110232
Scopus 85183359756



  • Phenotype, Biological Transport, Exosomes/metabolism, Biomarkers/metabolism, Extracellular Vesicles/metabolism