Mechanical Characterization of Micrometric Chitosan-Coated Vesicle by Atomic Force Microscopy

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • M. Rinaudo - , Université Grenoble Alpes (Author)
  • F. Quemeneur - , French Alternative Energies and Atomic Energy Commission (CEA), Institut Curie (Author)
  • F. Dubreuil - , Research Center for Plant Macromolecules (CERMAV) (Author)
  • A. Fery - , University of Bayreuth (Author)
  • B. Pépin-Donat - , French Alternative Energies and Atomic Energy Commission (CEA) (Author)

Abstract

This article reports the main results obtained for the stabilization of DOPC liposomes by coating with chitosan. We investigated the compression between two planes of isolated GUV, combining force spectroscopy measurements with scanning probe microscopy (SPM) and epifluorescence microscopy observations. We obtained reproducible and reversible force-deformation curves for individual vesicles in the range of small deformation (relative deformation up to 0.3). Force-deformation curves were analyzed using a simple elastic model, which well describes the observed radius-dependency of the force response and allows determining the stretching modulus for bare vesicle and effective bending and stretching moduli for chitosan-coated membranes. Results first show that chitosan coating increases the effective stretching modulus of the lipid membranes, second, confirm that chitosan is adsorbed flat on the membrane, and finally lead us to assume that the chitosan coating structure can be regarded as a physical network of connected chitosan patches.

Details

Original languageEnglish
Pages (from-to)617-626
Number of pages10
JournalInternational journal of polymer analysis and characterization : IJPAC
Volume18
Issue number8
Publication statusPublished - Nov 2013
Peer-reviewedYes
Externally publishedYes

Keywords

Keywords

  • AFM, DOPC lipid vesicle, Mechanical parameters, Mechanism of chitosan adsorption