Matrix Metalloproteinase 2 Polymorphisms in a Caucasian Population with Abdominal Aortic Aneurysm

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • I. Hinterseher - , TUD Dresden University of Technology (Author)
  • H. Bergert - , TUD Dresden University of Technology (Author)
  • E. Kuhlisch - , TUD Dresden University of Technology (Author)
  • A. Bloomenthal - , University of Massachusetts Medical School (Author)
  • Ch Pilarsky - , TUD Dresden University of Technology (Author)
  • D. Ockert - , TUD Dresden University of Technology (Author)
  • S. Schellong - , TUD Dresden University of Technology (Author)
  • H. D. Saeger - , TUD Dresden University of Technology (Author)
  • D. Krex - , Department of Neurosurgery (Author)

Abstract

Background: The formation of sporadic abdominal aortic aneurysm (AAA) is explained by remodeling of the extracellular matrix (ECM) and breakdown of structural components of the vascular wall. Matrix metalloproteinase 2 (MMP2) is one of the principal matrix-degrading proteases and is known to play a major role in the remodeling of the extracellular matrix in arterial vessels. Increased MMP2 expression in the extracellular matrix of the walls of AAAs has been shown in several studies. To investigate the possible impact of genetic variants of the MMP2 gene in the etiology of AAA, we conducted this case-control study. Patients and methods: We analyzed MMP2 single-nucleotide polymorphisms (SNPs) in 51 patients with AAA and 48 controls. Differences in genotype and allele frequencies of identified polymorphisms were determined after sequencing the entire coding region and three selected parts of the promoter. Results: Eighteen polymorphisms were identified, 6 of which are newly described, with 3 located in the introns (c.IVS1+31C>G, c.IVS7-18G>A, c.IVS10+26C>T) and 3 located in the coding region (c.124G>A, c.1368C>T, c.1860C>T). There were no statistically significant differences in genotype or allele frequencies between the two groups. Conclusions: Our analysis of the entire coding region and three parts of the promoter of the MMP2 gene failed to show an association between genetic polymorphisms and AAA, suggesting that variations in the MMP2 gene do not contribute to the development of AAA.

Details

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalJournal of Surgical Research
Volume133
Issue number2
Publication statusPublished - 15 Jun 2006
Peer-reviewedYes

External IDs

PubMed 16458924

Keywords

ASJC Scopus subject areas

Keywords

  • aneurysm, metalloproteinases, polymorphisms (genetics)