Markedly Different Effects of Monovalent Cations on the Efficiency of Gene Expression
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Contributors
Abstract
The effect of monovalent cations on a cell-free transcription–transla-
tion (TX–TL) system is examined using a luciferase assay. It is found that
the potency for all ions analyzed here is in the order Rb+ > K+ > Cs+ >
Na+ ≈ Li+ > (CH3)4N+, where Rb+ is most efficient at promoting TX–TL and the ions of Li+, Na+, and (CH3)4N+ exhibit an inhibitory effect. Similar promotion/ inhibition effects are observed for cell-free TL alone with an mRNA template.
tion (TX–TL) system is examined using a luciferase assay. It is found that
the potency for all ions analyzed here is in the order Rb+ > K+ > Cs+ >
Na+ ≈ Li+ > (CH3)4N+, where Rb+ is most efficient at promoting TX–TL and the ions of Li+, Na+, and (CH3)4N+ exhibit an inhibitory effect. Similar promotion/ inhibition effects are observed for cell-free TL alone with an mRNA template.
Details
| Original language | English |
|---|---|
| Article number | 2200164 |
| Number of pages | 8 |
| Journal | Advanced Biology |
| Volume | 7 |
| Issue number | 3 |
| Early online date | 3 Nov 2022 |
| Publication status | Published - Mar 2023 |
| Peer-reviewed | Yes |
External IDs
| Scopus | 85141436410 |
|---|---|
| ORCID | /0000-0001-8761-1776/work/122083677 |