Long‐term changes in the subgingival microbiota in patients with stage III–IV periodontitis treated by mechanical therapy and adjunctive systemic antibiotics: A secondary analysis of a randomized controlled trial

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Daniel Hagenfeld - , University Hospital Münster (First author)
  • Sven Kleine Bardenhorst - , University Hospital Münster (Author)
  • Johannes Matern - , University Hospital Münster (Author)
  • Karola Prior - , University Hospital Münster (Author)
  • Inga Harks - , University Hospital Münster (Author)
  • Peter Eickholz - , University Hospital Frankfurt (Author)
  • Katrin Lorenz - , Department of Periodontology (Author)
  • Ti‐Sun Kim - , University Hospital Heidelberg (Author)
  • Thomas Kocher - , University of Greifswald (Author)
  • Jörg Meyle - , University Hospital Gießen and Marburg (Author)
  • Doğan Kaner - , Charité – Universitätsmedizin Berlin (Author)
  • Ulrich Schlagenhauf - , University Hospital of Würzburg (Author)
  • Dag Harmsen - , University Hospital Münster (Author)
  • Benjamin Ehmke - , University Hospital Münster (Last author)

Abstract

Aim: To explore whether adjunctive antibiotics can relevantly influence long-term microbiota changes in stage III-IV periodontitis patients.

Materials and methods: This is a secondary analysis of a randomized clinical trial on periodontal therapy with adjunctive 500 mg amoxicillin and 400 mg metronidazole or placebo thrice daily for 7 days. Subgingival plaque samples were taken before and 2, 8, 14 and 26 months after mechanical therapy. The V4-hypervariable region of the 16S rRNA gene was sequenced with Illumina MiSeq 250 base pair paired-end reads. Changes at the ribosomal sequence variant (RSV) level, diversity and subgingival-microbial dysbiosis index (SMDI) were explored with a negative binomial regression model and non-parametric tests.

Results: Overall, 50.2% of all raw reads summed up to 72 RSVs (3.0%) that were generated from 163 stage III-IV periodontitis patients. Of those, 16 RSVs, including Porphyromonas gingivalis, Tannerella forsythia and Aggregatibacter actinomycetemcomitans, changed significantly over 26 months because of adjunctive systemic antibiotics. SMDI decreased significantly more in the antibiotic group at all timepoints, whereas the 2-month differences in alpha and beta diversity between groups were not significant at 8 and 14 months, respectively.

Conclusions: Mechanical periodontal therapy with adjunctive antibiotics induced a relevant and long-term sustainable change towards an oral microbiome more associated with oral health.

Details

Original languageEnglish
Pages (from-to)1101-1112
Number of pages12
JournalJournal of clinical periodontology
Volume50 (2023)
Issue number8
Publication statusPublished - 13 Jul 2023
Peer-reviewedYes

External IDs

Scopus 85158951780
Mendeley bfbe6347-d82c-39bc-8b27-eaef122d8761

Keywords

Research priority areas of TU Dresden

Keywords

  • clinical trial, therapy, microbiology, periodontal diseases

Library keywords