Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Dirk Haufe - , University Hospital Carl Gustav Carus Dresden, Department of Anesthesiology and Intensive Care Medicine (Author)
  • Eva Koenigshausen - , University Hospital Carl Gustav Carus Dresden, Department of Anesthesiology and Intensive Care Medicine (Author)
  • Lilla Knels - , University Hospital Carl Gustav Carus Dresden, Department of Anesthesiology and Intensive Care Medicine, Institute of Anatomy (Author)
  • Martina Wendel - , University Hospital Carl Gustav Carus Dresden, Department of Anesthesiology and Intensive Care Medicine (Author)
  • Sebastian N. Stehr - , University Hospital Carl Gustav Carus Dresden, Department of Anesthesiology and Intensive Care Medicine (Author)
  • Thea Koch - , Department of Anesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus Dresden (Author)

Abstract

Application of liquid, aerosolized, and vaporized perfluorocarbons (PFC) in acute lung injury has shown anti-inflammatory effects. Although this may be beneficial in states of pulmonary hyperinflammation, it also could increase susceptibility to nosocomial lung infection. We hypothesized that PFC impair cellular host defense and therefore investigated in an in vitro model the influence of perfluorohexane (PFH) on crucial mechanisms of bacterial elimination in human neutrophils and monocytes. Using scanning and transmission electron microscopy, we could show membrane-bound and ingested PFH particles that morphologically did not alter adherence and phagocytosis of Escherichia coli or leukocyte viability. The amount of adherent and phagocytosed bacteria as determined by flow cytometry was not influenced in cells only pretreated with PFH for 1 and 4 h. When PFH was present during E. coli challenge, bacterial adherence was decreased in polymorphonuclear neutrophils, but respective intracellular uptake was not impaired and was even significantly promoted in monocytes. Overall, E. coli-induced respiratory burst capacity was not reduced by PFH. Our findings provide evidence that key functions of innate host defense are not compromised by PFH treatment in vitro.

Details

Original languageEnglish
Pages (from-to)L134-L142
JournalAmerican journal of physiology - Lung cellular and molecular physiology
Volume295
Issue number1
Publication statusPublished - Jul 2008
Peer-reviewedYes

External IDs

PubMed 18456798

Keywords

Keywords

  • Anti-inflammatory, Burst, Immune, Perfluorohexane, Phagocytosis