Kinetics and functional characterization of the glycine receptor α2 and α3 subunit

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Cornelia Heindl - , Friedrich-Alexander University Erlangen-Nürnberg (Author)
  • Kay Brune - , Friedrich-Alexander University Erlangen-Nürnberg (Author)
  • Bertold Renner - , Institute of Clinical Pharmacology, Friedrich-Alexander University Erlangen-Nürnberg (Author)

Abstract

In recent studies one serine residue (Ser-346) within the protein-kinase-A (R-E-S-R) consensus sequence of the GlyRα3 intracellular loop has proven to be an essential target for prostaglandin-E2-mediated phosphorylation, which further modulates spinal nociceptive transmission and central inflammatory pain sensitization. In the present study we investigated the effect of Ser-346 phosphorylation and Ser-346 mutation on receptor kinetics and function using whole-cell patch-clamp recordings in transfected HEK 293 T cells. We compared biophysical properties of wild type GlyRα3 and two site-directed mutants, where Ser-346 was replaced by alanine or aspartate, in the absence and presence of prostaglandin-E2. The mutation to alanine was accompanied by significantly altered dose-response and desensitization properties. Mutation to aspartate had only minor effects on receptor kinetics and function. Phosphorylation of Ser-346 slowed desensitization and decreased glycinergic currents in GlyRα3/mEP2 transfected cells. In addition, we demonstrated that prostaglandin-E2 also had an effect on the GlyRα2 subunit. Exposure to prostaglandin-E2 decreased the maximum peak current amplitude of glycinergic currents in GlyRα2/mEP2 transfected cells in the same manner as phosphorylation of the GlyRα3 subunit. It led to a significant increase of the desensitization time constants and thus significantly affected the desensitization behaviour. These results indicate that the GlyRα2 and the GlyRα3 subunits act as important subunits for the modulation of glycine receptor kinetics and function.

Details

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalNeuroscience letters
Volume429
Issue number1
Publication statusPublished - 11 Dec 2007
Peer-reviewedYes

External IDs

PubMed 17997221
ORCID /0000-0003-0845-6793/work/139025193

Keywords

ASJC Scopus subject areas

Keywords

  • Desensitization, Glycine receptor α2 subunit, Glycine receptor α3 subunit, Intracellular loop, Kinetics, Phosphorylation, Serine