Isolation of polymorphic microsatellite loci in three phantom midge species of the genus Chaoborus (Diptera: Chaoboridae)

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • T. U. Berendonk - , Max Planck Institute for Evolutionary Biology, NERC Centre for Population Biology, Leipzig University (Author)
  • T. Evans - , NERC Centre for Population Biology (Author)

Abstract

Because of its widespread distribution in lakes and ponds Chaoborus is of great interest to many freshwater ecologists. Interestingly some species are restricted to small fish-less water bodies, whereas other species live mostly in large lakes. To eventually test the genetic and evolutionary implications of these different lifestyles we identified microsatellite loci in three species in this preliminary study: C. obscuripes, C. crystallinus and C. flavicans. Using a biotin/streptavidin capture technique of repetitive sequences in a 96 well format, we obtained microsatellite-enriched genomic libraries for all three species and identified six polymorphic microsatellite markers for each species.

Details

Original languageEnglish
Pages (from-to)250-252
Number of pages3
JournalMolecular Ecology Notes
Volume4
Issue number2
Publication statusPublished - Jun 2004
Peer-reviewedYes
Externally publishedYes

External IDs

ORCID /0000-0002-9301-1803/work/161409790

Keywords

Keywords

  • C. crystallinus, C. flavicans, C. obscuripes, Chaoborus, Freshwater, Microsatellites