Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages
Research output: Contribution to book/conference proceedings/anthology/report › Chapter in book/anthology/report › Contributed › peer-review
Contributors
Abstract
Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.
Details
Original language | English |
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Title of host publication | Tissue-Resident Macrophages |
Publisher | Springer Nature |
Pages | 231-251 |
Number of pages | 21 |
Publication status | Published - 29 Aug 2023 |
Peer-reviewed | Yes |
Publication series
Series | Methods in Molecular Biology (MIMB; Vol. 2713) |
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Volume | 2713 |
ISSN | 1064-3745 |
External IDs
Scopus | 85168852873 |
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Keywords
Keywords
- Animals, Mice, Macrophages, Alveolar, Macrophages, Thorax