Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportContributedpeer-review

Contributors

Abstract

Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.

Details

Original languageEnglish
Title of host publicationTissue-Resident Macrophages
PublisherSpringer Nature
Pages231-251
Number of pages21
Publication statusPublished - 29 Aug 2023
Peer-reviewedYes

Publication series

SeriesMethods in Molecular Biology (MIMB; Vol. 2713)
Volume2713
ISSN1064-3745

External IDs

Scopus 85168852873

Keywords

Keywords

  • Animals, Mice, Macrophages, Alveolar, Macrophages, Thorax

Library keywords