Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

Clara Jana-Lui Busch; Sethuraman Subramanian; Javier Linares; Jérémy Favret; Ridzky Anis Advent Yuda; Michael H Sieweke

doi:10.1007/978-1-0716-3437-0_16

Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

Research output: Contribution to book/conference proceedings/anthology/report › Chapter in book/anthology/report › Contributed › peer-review

Contributors

Clara Jana-Lui Busch - , Center for Regenerative Therapies Dresden (CRTD) (Author)
Sethuraman Subramanian - , Chair of Stem Cell Research with focus on cell-based approaches to regenerative biomedicine (Author)
Javier Linares - , Chair of Stem Cell Research with focus on cell-based approaches to regenerative biomedicine (Author)
Jérémy Favret - , Center for Regenerative Therapies Dresden (CRTD) (Author)
Ridzky Anis Advent Yuda - , Chair of Stem Cell Research with focus on cell-based approaches to regenerative biomedicine (Author)
Michael H Sieweke - , Chair of Stem Cell Research with focus on cell-based approaches to regenerative biomedicine, Aix-Marseille Université (Author)

Abstract

Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.

Details

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Springer Nature
Pages	231-251
Number of pages	21
Volume	2713
Publication status	Published - 2024
Peer-reviewed	Yes

Publication series

Series	Methods in molecular biology (Clifton, N.J.)
ISSN	1064-3745

External IDs

Scopus	85168852873

Keywords

Animals, Mice, Macrophages, Alveolar, Macrophages, Thorax

Research Portal of the TU Dresden

Contributors

Abstract

Details

Publication series

External IDs

Keywords

Keywords