This article presents an image-based analysis of cork cells from two cork rings within the same cork profile using a scanning electron microscope. The study also examines cork characteristics in relation to cork ring width and ring density. During the cork growth cycle, forest management practices were altered due to the additional irrigation applied to the plot. The objective of this study is to assess changes in cork cell walls and lumens’ response to the additional irrigation by following their previously documented growth pattern or by deviations as a consequence of the altered forest management approach. Cellular analyses showed that both the year of the growth ring and the treatment influenced the measurements. In particular, thicker cell walls were observed in the 2022 ring under irrigation conditions. However, the results showed no statistically significant differences in overall cork growth between treatments.