Interferon but not MxB inhibits foamy retroviruses

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Ariane Bähr - , Heinrich Heine University Düsseldorf (Author)
  • Anna Singer - , Heinrich Heine University Düsseldorf (Author)
  • Anika Hain - , Heinrich Heine University Düsseldorf (Author)
  • Ananda Ayyappan Jaguva Vasudevan - , Heinrich Heine University Düsseldorf (Author)
  • Mirjam Schilling - , University Medical Center Freiburg (Author)
  • Juliane Reh - , Institute of Medical Microbiology and Virology (Author)
  • Maximilian Riess - , Paul-Ehrlich-Institut (Author)
  • Sylvia Panitz - , Paul-Ehrlich-Institut (Author)
  • Vanessa Serrano - , Sanford Burnham Prebys Medical Discovery Institute (Author)
  • Matthias Schweizer - , Paul-Ehrlich-Institut (Author)
  • Renate König - , Paul-Ehrlich-Institut (Author)
  • Sumit Chanda - , Sanford Burnham Prebys Medical Discovery Institute (Author)
  • Dieter Häussinger - , Heinrich Heine University Düsseldorf (Author)
  • Georg Kochs - , University Medical Center Freiburg (Author)
  • Dirk Lindemann - , Institute of Medical Microbiology and Virology, Center for Regenerative Therapies Dresden (Author)
  • Carsten Münk - , Heinrich Heine University Düsseldorf (Author)

Abstract

Foamy viruses (FV) are retroviruses that are widely distributed in primate and non-primate animal species. We tested here FV with capsids of simian and non-simian origin for sensitivity to interferon-β (IFN-β). Our data show significant inhibition of FV by IFN-β early in infection of human HOS and THP-1 but not of HEK293T cells. The post-entry restriction of FV was not mediated by the interferon-induced MxB protein that was recently identified as a capsid-interacting restriction factor targeting Human immunodeficiency virus (HIV) before integration. Neither the ectopic expression of MxA or MxB in HEK293T cells nor the lack of MxB expression in CRISPR/CAS MxB THP-1 knockout cells impacted the infection of the tested FV. IFN-β treated THP-1 and THP-1 KO MxB cells showed the same extend of restriction to FV. Together, the data demonstrate that IFN-β inhibits FV early in infection and that MxB is not a restriction factor of FV.

Details

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalVirology
Volume488
Publication statusPublished - 15 Jan 2016
Peer-reviewedYes

External IDs

ORCID /0000-0002-0320-4223/work/150885015
Scopus 84947792510

Keywords

Keywords

  • Cell Line, Humans, Interferon-beta/metabolism, Myxovirus Resistance Proteins/deficiency, Spumavirus/immunology