Interactions of bioactive molecules with thin dendritic glycopolymer layers
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The authors report on highly swellable, stable layers of spherical dendritic glycopolymers, composed of hyperbranched poly(ethylene imine) (PEI) as core and two different maltose shells (A = dense shell and B = open shell). These glycopolymers are cross-linked and attached with poly(ethylene-alt-maleic anhydride) (PEMA) or citric acid on SiOx substrates. The swelling and adsorption of biomolecules were analyzed by spectroscopic ellipsometry and quartz crystal microbalance with dissipation. The swelling degree and complexation with the drug molecule adenosine triphosphate (ATP) were found to be up to 10 times higher for dendritic glycopolymer layers cross-linked with PEMA than for layers cross-linked with citric acid. ATP complexation by electrostatic interaction with the PEI cores was confirmed by x-ray photoelectron spectroscopy analysis. Complexation led to partial collapsing, stiffening, and increase of polymer layer viscosity of the PEMA cross-linked layers. From modeling of ellipsometric data, it was deduced that ATP complexation preferably takes place at the polymer layer-solution interface. The size effect of the adsorbates was investigated by comparing ATP complexation with the adsorption of larger vitamin B12 and human serum albumin (HSA) protein. PEI-Mal A cross-linked with PEMA was found to be resistant toward B12 and HSA adsorption due to the diffusion barrier of the polymer layer. Thus, the authors present potentially biocompatible polymer surfaces for drug loading and their surface supported release. Published by the AVS.
Details
Original language | German |
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Number of pages | 15 |
Journal | Biointerphases |
Volume | 13 |
Issue number | 6 |
Publication status | Published - 2018 |
Peer-reviewed | Yes |
External IDs
PubMed | 30227717 |
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Scopus | 85053735503 |
ORCID | /0000-0002-4531-691X/work/148608060 |
Keywords
Keywords
- Quartz-crystal microbalance, Human-serum-albumin, Pvfa-co-pvam, Poly(ethylene imine), Protein adsorption, Polyelectrolyte multilayers, Polymer capsules, Drug-delivery, Poly(propyleneimine) dendrimers, Polyamidoamine dendrimers