Influence of Pre-Analytic Conditions on Quantity of Lymphocytes

Research output: Contribution to journalResearch articleContributedpeer-review



Lymphocytes are key players in the pathogenesis of multiple sclerosis and a distinct target of several immunomodulatory treatment strategies. In this study, we aim to evaluate the effect of various pre-analytic conditions on immune cell counts to conclude the relevance for clinical implications. Twenty healthy donors were assessed for the effects of distinct storage temperatures and times after blood draws, different durations of tourniquet application, body positions and varying aspiration forces during blood draws. Immune cell frequencies were analyzed using multicolor flowcytometry. While storage for 24 h at 37 °C after blood draws was associated with significantly lower cell counts, different durations of tourniquet application, body positions and varying aspirations speeds did not have significant impacts on the immune cell counts. Our data suggest that immune cell counts are differently affected by pre-analytic conditions being more sensitive to storage temperature. Pre-analytic conditions should be carefully considered when interpreting the laboratory values of immune cell subpopulations.


Original languageEnglish
Article number13479
JournalInternational journal of molecular sciences
Issue number17
Publication statusPublished - 30 Aug 2023

External IDs

Scopus 85170250327
PubMed 37686285
Mendeley b9038d65-e46d-364d-b188-5f74f9668fec
WOS 001060644900001



  • Cell Count, Flow Cytometry, Health Status, Immunomodulation, Lymphocytes, autoimmune diseases, flowcytometry, pre-analytic conditions, lymphocytes, storage time, storage temperature, Flowcytometry, Storage time, Storage temperature, Autoimmune diseases, Pre-analytic conditions