Induced pluripotent stem cells of patients with Tetralogy of Fallot reveal transcriptional alterations in cardiomyocyte differentiation

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Marcel Grunert - , Charité – Universitätsmedizin Berlin, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Author)
  • Sandra Appelt - , Charité – Universitätsmedizin Berlin, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Author)
  • Sophia Schönhals - , Charité – Universitätsmedizin Berlin, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), Free University of Berlin (Author)
  • Kerstin Mika - , Charité – Universitätsmedizin Berlin (Author)
  • Huanhuan Cui - , Charité – Universitätsmedizin Berlin, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Author)
  • Ashley Cooper - , Charité – Universitätsmedizin Berlin (Author)
  • Lukas Cyganek - , University of Göttingen (Author)
  • Kaomei Guan - , Institute of Pharmacology and Toxicology, University Medical Center Göttingen, University of Göttingen, TUD Dresden University of Technology (Author)
  • Silke R. Sperling - , Charité – Universitätsmedizin Berlin, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), Free University of Berlin (Author)

Abstract

Patient-specific induced pluripotent stem cells (ps-iPSCs) and their differentiated cell types are a powerful model system to gain insight into mechanisms driving early developmental and disease-associated regulatory networks. In this study, we use ps-iPSCs to gain insights into Tetralogy of Fallot (TOF), which represents the most common cyanotic heart defect in humans. iPSCs were generated and further differentiated to cardiomyocytes (CMs) using standard methods from two well-characterized TOF patients and their healthy relatives serving as controls. Patient-specific expression patterns and genetic variability were investigated using whole genome and transcriptome sequencing data. We first studied the clonal mutational burden of the derived iPSCs. In two out of three iPSC lines of patient TOF-01, we found a somatic mutation in the DNA-binding domain of tumor suppressor P53, which was not observed in the genomic DNA from blood. Further characterization of this mutation showed its functional impact. For patient TOF-02, potential disease-relevant differential gene expression between and across cardiac differentiation was shown. Here, clear differences at the later stages of differentiation could be observed between CMs of the patient and its controls. Overall, this study provides first insights into the complex molecular mechanisms underlying iPSC-derived cardiomyocyte differentiation and its transcriptional alterations in TOF.

Details

Original languageEnglish
Article number10921
JournalScientific reports
Volume10
Issue number1
Publication statusPublished - 1 Dec 2020
Peer-reviewedYes

External IDs

PubMed 32616843

Keywords

ASJC Scopus subject areas