In vitro blood compatibility of polymeric biomaterials through covalent immobilization of an amidine derivative
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
We present a surface coating with anticoagulant characteristics showing significantly reduced coagulation activation. The synthesis of a monomeric conjugate containing a benzamidine moiety was carried out and its inhibitory activity against human thrombin, the key enzyme of the blood coagulation cascade, was determined using a chromogenic assay. Based on that, low-thrombogenic interfaces were prepared by covalent attachment of this low-molecular weight thrombin inhibitor on poly(octadecene-alt-maleic anhydride) copolymer thin films and characterized using ellipsometry, XPS and dynamic contact angle measurements. The in vitro hemocompatibility tests using freshly drawn human whole blood showed, in agreement with the SEM images, that a PO-MA film modified with a benzamidine moiety using a PEG spacer decreased the activation of coagulation, platelets and the complement system. The decreased protein adsorption, in addition to the specific inhibition of thrombin, effectively enhanced the short-term hemocompatibility characteristics. (C) 2003 Elsevier Ltd. All rights reserved.
Details
Original language | English |
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Pages (from-to) | 3493-3501 |
Number of pages | 9 |
Journal | Biomaterials |
Volume | 25 |
Issue number | 17 |
Publication status | Published - Aug 2004 |
Peer-reviewed | Yes |
External IDs
PubMed | 15020123 |
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Scopus | 12144289575 |
ORCID | /0000-0002-4531-691X/work/148607690 |
ORCID | /0000-0003-0189-3448/work/159607202 |
Keywords
Keywords
- Blood compatibility, Immobilization, Maleic anhydride copolymer, Surface coating, Thrombin inhibitor