Wound infection monitoring is a matter of high interest in wound treatment. For this purpose, we extracted sensor parameters suitable to identify massive pathogen presence. One method is to measure neutrophil-derived extracellular DNA as an immediate response of the immune system. We proofed that impedance measurement is a reasonable measurement technique for this parameter. Our sensor concept comprises a planar or fiber-based set-up integrable into a wound cover. Proof-of-principle experiments using impedance spectroscopy in cell cultures showed a dependency correlation between extracellular DNA formation and impedance rise. This was verified by microscopic observation of fluorescently stained samples. A reproducible impedance signal deviation of in average 34% up to 4 hours later at most was observed.