Immune Surveillance of Acute Myeloid Leukemia Is Mediated by HLA-Presented Antigens on Leukemia Progenitor Cells
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
UNLABELLED: Therapy-resistant leukemia stem and progenitor cells (LSC) are a main cause of acute myeloid leukemia (AML) relapse. LSC-targeting therapies may thus improve outcome of patients with AML. Here we demonstrate that LSCs present HLA-restricted antigens that induce T-cell responses allowing for immune surveillance of AML. Using a mass spectrometry-based immunopeptidomics approach, we characterized the antigenic landscape of patient LSCs and identified AML- and AML/LSC-associated HLA-presented antigens absent from normal tissues comprising nonmutated peptides, cryptic neoepitopes, and neoepitopes of common AML driver mutations of NPM1 and IDH2. Functional relevance of shared AML/LSC antigens is illustrated by presence of their cognizant memory T cells in patients. Antigen-specific T-cell recognition and HLA class II immunopeptidome diversity correlated with clinical outcome. Together, these antigens shared among AML and LSCs represent prime targets for T cell-based therapies with potential of eliminating residual LSCs in patients with AML.
SIGNIFICANCE: The elimination of therapy-resistant leukemia stem and progenitor cells (LSC) remains a major challenge in the treatment of AML. This study identifies and functionally validates LSC-associated HLA class I and HLA class II-presented antigens, paving the way to the development of LSC-directed T cell-based immunotherapeutic approaches for patients with AML. See related commentary by Ritz, p. 430 . This article is featured in Selected Articles from This Issue, p. 419.
Details
Original language | English |
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Pages (from-to) | 468-489 |
Number of pages | 22 |
Journal | Blood cancer discovery |
Volume | 4 |
Issue number | 6 |
Publication status | Published - 1 Nov 2023 |
Peer-reviewed | Yes |
External IDs
PubMedCentral | PMC10618727 |
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unpaywall | 10.1158/2643-3230.bcd-23-0020 |
Scopus | 85177494937 |
Keywords
Keywords
- Humans, HLA Antigens, Leukemia, Myeloid, Acute/genetics, Peptides, Stem Cells