Identification of Epigenetically Regulated Genes Distinguishing Intracranial from Extracranial Melanoma Metastases

Research output: Contribution to journalResearch articleContributedpeer-review



Despite remarkable advances in treating patients with metastatic melanoma, the management of melanoma brain metastases remains challenging. Recent evidence suggests that epigenetic reprogramming is an important mechanism for the adaptation of melanoma cells to the brain environment. In this study, the methylomes and transcriptomes of a cohort of matched melanoma metastases were evaluated by integrated omics data analysis. The identified 38 candidate genes displayed distinct promoter methylation and corresponding gene expression changes in intracranial compared with extracranial metastases. The 11 most promising genes were validated on protein level in both tumor and surrounding normal tissue using immunohistochemistry. In accordance with the underlying promoter methylation and gene expression changes, a significantly different protein expression was confirmed for STK10, PDXK, WDR24, CSSP1, NMB, RASL11B, phosphorylated PRKCZ, PRKCZ, and phosphorylated GRB10 in the intracranial metastases. The observed changes imply a distinct intracranial phenotype with increased protein kinase B phosphorylation and a higher frequency of proliferating cells. Knockdown of PRKCZ or GRB10 altered the expression of phosphorylated protein kinase B and decreased the viability of a brain-specific melanoma cell line. In summary, epigenetically regulated cancer-relevant alterations were identified that provide insights into the molecular mechanisms that discriminate brain metastases from other organ metastases, which could be exploited by targeting the affected signaling pathways.


Original languageEnglish
Pages (from-to)1233-1245.e17
Number of pages30
JournalJournal of Investigative Dermatology
Issue number7
Early online date27 Jan 2023
Publication statusPublished - Jul 2023

External IDs

ORCID /0000-0001-7499-5125/work/130201217
unpaywall 10.1016/j.jid.2023.01.011
Scopus 85149672475
PubMed 36716920
ORCID /0000-0001-5130-6964/work/142249510
ORCID /0000-0003-4340-9706/work/143497450
ORCID /0000-0003-4340-0402/work/145223793
ORCID /0000-0002-2844-053X/work/153110481


Research priority areas of TU Dresden

Sustainable Development Goals


  • Brain Neoplasms/genetics, Brain/metabolism, Humans, Melanoma/pathology, Monomeric GTP-Binding Proteins/metabolism, Protein Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins c-akt/metabolism

Library keywords