Hydrogel-Coated Nanonet-Based Field-Effect Transistors for SARS-CoV-2 Spike Protein Detection in High Ionic Strength Samples
Research output: Contribution to book/Conference proceedings/Anthology/Report › Conference contribution › Contributed › peer-review
Contributors
Abstract
The SARS-CoV-2 pandemic has triggered many studies worldwide in the area of biosensors, leading to innovative approaches for the quantitative assessment of COVID-19. A nanostructured field-effect transistor (FET) is one type of device shown to be ultrasensitive for virus determination. FETs can be used as transducers to analyze changes in electrical current caused by the bonding of viral molecules to the surface of the semiconducting nanomaterial layer of the FETs. Although nano-transistors require simple setups amenable to be miniaturized for point-of-care diagnostic of COVID-19, this type of sensor usually has limited sensitivity in biological fluids. The reason behind this is the shortened screening length in the presence of high ionic strength solutions. In the frame of this study, we propose a methodology consisting of the FET surface modification with a hydrogel based on the star-shaped polyethylene glycol (starPEG), which hosts specific antibodies against SARS-CoV-2 spike protein in its porous structure. The deposition of the hydrogel increases the effective Debye length, preserving the biosensor’s sensitivity. We demonstrate the capability of silicon nanonet-based FETs to detect viral antigens and cultured viral particles in phosphate-buffered saline (PBS) as well as in human-purified saliva. Finally, we discriminated between positive and negative patients’ nasopharyngeal swab samples.
Details
Original language | English |
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Title of host publication | Hydrogel-Coated Nanonet-Based Field-Effect Transistors for SARS-CoV-2 Spike Protein Detection in High Ionic Strength Samples |
Volume | 35 |
Edition | 1 |
Publication status | Published - 8 May 2023 |
Peer-reviewed | Yes |
External IDs
unpaywall | 10.3390/iecb2023-14566 |
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ORCID | /0000-0002-9899-1409/work/142249233 |
Scopus | 85172771982 |
ORCID | /0000-0003-0189-3448/work/159607199 |
ORCID | /0000-0001-8893-5326/work/173988540 |
Keywords
Research priority areas of TU Dresden
DFG Classification of Subject Areas according to Review Boards
- Theoretical Chemistry: Molecules, Materials, Surfaces
- Theoretical Chemistry: Electron Structure, Dynamics, Simulation
- Theoretical Condensed Matter Physics
- Statistical Physics, Soft Matter, Biological Physics, Nonlinear Dynamics
- Thermodynamics and Kinetics as well as Properties of Phases and Microstructure of Materials
- Biomaterials
- Computer-aided Material Design and Simulation of Material Behaviour from Atomistic to Microscopic Scale
- Synthesis and Properties of Functional Materials
- Experimental Condensed Matter Physics
- Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry
Subject groups, research areas, subject areas according to Destatis
- Optoelectronics
- Micro- and Nanoelectronics
- Theoretical Physics
- Sensors and Measurement Technology
- Software Technology
- Solid State Physics
- Materials Science
- Virology
- Materials Physics
- Forensic Medicine
- Library Science (general)
- Biomedical Engineering
- Building Materials Technology
- Environmental Engineering (incl. Recycling)
Sustainable Development Goals
- SDG 17 - Partnerships for the Goals
- SDG 7 - Affordable and Clean Energy
- SDG 6 - Clean Water and Sanitation
- SDG 9 - Industry, Innovation, and Infrastructure
- SDG 15 - Life on Land
- SDG 5 - Gender Equality
- SDG 1 - No Poverty
- SDG 11 - Sustainable Cities and Communities
- SDG 13 - Climate Action
- SDG 3 - Good Health and Well-being
- SDG 12 - Responsible Consumption and Production
ASJC Scopus subject areas
Keywords
- COVID-9 diagnostics, Debye screening length, field-effect transistor, hydrogel biosensor, SARS-CoV-2 detection, starPEG