Hes3 expression in the adult mouse brain is regulated during demyelination and remyelination

Research output: Contribution to journalResearch articleContributedpeer-review



Hes3 is a component of the STAT3-Ser/Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells. Pharmacological activation of this pathway promotes neuronal rescue and behavioral recovery in models of ischemic stroke and Parkinson's disease. Here we prov ide initial observations implicating Hes3 in the cuprizone model of demyelination and remyelination. We focus on the subpial motor cortex of mice because we detected high Hes3 expression. This area is of interest as it is impacted both in human demyelinating diseases and in the cuprizone model. We report that Hes3 expression is reduced at peak demyelination and is partially restored within 1 week after cuprizone withdrawal. This raises the possibility of Hes3 involvement in demyelination/remyelination that may warrant additional research. Supporting a possible role of Hes3 in the maintenance of oligodendrocyte markers, a Hes3 null mouse strain shows lower levels of myelin basic protein in undamaged adult mice, compared to wild-type controls. We also present a novel method for culturing the established oligodendrocyte progenitor cell line oli-neu in a manner that maintains Hes3 expression as well as its self-renewal and differentiation potential, offering an experimental tool to study Hes3. Based upon this approach, we identify a Janus kinase inhibitor and dbcAMP as powerful inducers of Hes3 gene expression. We provide a new biomarker and cell culture method that may be of interest in demyelination/remyelination research.


Original languageEnglish
Pages (from-to)124-130
Number of pages7
JournalMolecular Brain Research
Publication statusPublished - 1 Jul 2016

External IDs

researchoutputwizard legacy.publication#72761
Scopus 84962653794
PubMed 27018293
researchoutputwizard legacy.publication#72991


Sustainable Development Goals


  • Brain, Hes3, Myelin, Oligodendrocyteprecursor, Regeneration