Drawing on the challenge-hindrance stressor framework and the job demands-control-support model, this study investigates the moderating role of goal clarity and peer support within the relationships of challenge and hindrance stressors with job stress. While conducting an experience sampling study, and analyzing 624 observations made by 66 early-career professionals with hierarchical linear modeling (HLM), our results show positive direct effects of both challenge and hindrance stressors on perceived job stress. Moreover, our results from the multilevel interaction analyses show the relationship between challenge stressors and perceived job stress to vary depending on the level of goal clarity, and the respective relationship of hindrance stressors to be contingent upon the respondents' peer support. In contrast, the proposed buffering role of goal clarity in the relationship between hindrance stressors and strain, just as the buffering role of peer support in the challenge stressor–strain relationship were not supported. Thus, our study entails valuable implications, which are discussed.