Genotoxicity of poly(propylene imine) dendrimers

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Barbara Ziemba - , Lodz University of Technology (Author)
  • Gabriela Matuszko - , Lodz University of Technology (Author)
  • Dietmar Appelhans - , Leibniz Institute of Polymer Research Dresden (Author)
  • Brigitte Voit - , Leibniz Institute of Polymer Research Dresden (Author)
  • Maria Bryszewska - , Lodz University of Technology (Author)
  • Barbara Klajnert - , Lodz University of Technology (Author)

Abstract

Dendrimers are highly branched macromolecules with the potential in biomedical applications. Due to positively charged surfaces, several dendrimers reveal toxicity. Coating peripheral cationic groups with carbohydrate residues can reduce it. In this study, the cytotoxicity and genotoxicity of three types of 4th generation poly(propylene imine) dendrimers were investigated. Peripheral blood mononuclear cells (PBMCs) were treated with uncoated (PPI-g4) dendrimers, and dendrimers in which approximately 40% or 90% of peripheral amino groups were coated with maltotriose (PPI-g4-OS or PPI-g4-DS) at concentration of 0.05, 0.5, 5 mg/ml. Abbreviations OS and DS stand for open shell and dense shell respectively, that describes the structure of carbohydrate modified dendrimers. After 1 h of cell incubation at 37 degrees C, the MTT and comet assays were performed. PPI dendrimers demonstrated surface-modification-degree dependent toxicity, although genotoxicity of PPI-g4 and PPI-g4-OS measured by the comet assay was concentration dependent up to 0.5 mg/ml and at 5 mg/ml the amount of DNA that left comet's head decreased. Results may suggest a strong interaction between dendrimers and DNA, and furthermore, that coating PPI dendrimers by maltoriose is an efficient method to reduce their genotoxicity what opens the possibilities to use them as therapeutic agents or drug carriers. (c) 2012 Wiley Periodicals, Inc. Biopolymers 97: 642648, 2012.

Details

Original languageEnglish
Pages (from-to)642-648
Number of pages7
JournalBiopolymers
Volume97
Issue number8
Publication statusPublished - Aug 2012
Peer-reviewedYes
Externally publishedYes

External IDs

PubMed 22605555
Scopus 84861309594
ORCID /0000-0002-4531-691X/work/148607864

Keywords

Keywords

  • Ppi, Comet assay, Dendrimer, Genotoxicity, Glycodendrimers