Genomic characterization of blaVIM-11-harbouring plasmids recovered from Pseudomonas aeruginosa

Research output: Contribution to journalComment/DebateContributedpeer-review

Contributors

  • Alan Elena - , Chair of Limnology, Universidad de Buenos Aires, National Scientific and Technical Research Council Argentina (CONICET) (Author)
  • Daniela Cejas - , Universidad de Buenos Aires, National Scientific and Technical Research Council Argentina (CONICET) (Author)
  • Gabriel Gutkind - , Universidad de Buenos Aires, National Scientific and Technical Research Council Argentina (CONICET) (Author)
  • Marcela Radice - , Universidad de Buenos Aires, National Scientific and Technical Research Council Argentina (CONICET) (Author)

Abstract

Objectives: To the best of our knowledge, no genomic descriptions of blaVIM-11-harbouring plasmids are available in literature so far. The aim of this study was to describe the genomic features of three blaVIM-11-harbouring plasmids recovered from Pseudomonas aeruginosa isolated in Argentina in different periods. Methods: blaVIM-11-harbouring plasmids from three clinical P. aeruginosa isolates were transferred by transformation into P. aeruginosa PAO-1. Then, genomic DNA of these transformants was extracted and sequenced using NovaSeq 6000 System-Illumina. De novo assemblies were generated using Unicycler program and reads were mapped against a reference genome of P. aeruginosa PAO-1. Plasmids sequences were predicted identifying the reads that did not map the reference sequence of PAO-1. These reads were recovered and assembled de novo. In silico predictions were carried out using bioinformatics tools. Results: One Plasmid (pP6VIM-11) was distributed in 2 contigs, a second plasmid (pPOta2VIM-11) was found in a single contig, and the last one (pP936401VIM-11) was fragmented into 4 contigs. pP6VIM-11 and pPOta2VIM-11 belonged to the IncP-1β group, displaying 64% of coverage and 83.9% of identity among them. pP936401VIM-1 plasmid corresponded to the IncN group. The bioinformatic analysis revealed that blaVIM-11 was located in a class 1 integron, flanked by insertion sequences, exhibiting potential for its dissemination. However, none of the plasmids were conjugative. Conclusion: This study corresponded to the first description and deposit of blaVIM-11-harbouring plasmids in P. aeruginosa, which expands the limited knowledge about their molecular epidemiology.

Details

Original languageEnglish
Pages (from-to)190-192
Number of pages3
JournalJournal of global antimicrobial resistance
Volume36
Publication statusPublished - Mar 2024
Peer-reviewedYes

External IDs

PubMed 38154753
ORCID /0000-0001-5372-0923/work/152544435

Keywords

Keywords

  • Argentina, IncN, IncP1, Plasmids, Pseudomonas aeruginosa, VIM-11, Genomics, Plasmids/genetics, Pseudomonas aeruginosa/genetics, beta-Lactamases/genetics, DNA, Bacterial/genetics