Genetically Encoded Sensors to Study Metabolism in Drosophila
Research output: Contribution to book/Conference proceedings/Anthology/Report › Chapter in book/Anthology/Report › Invited › peer-review
Contributors
Abstract
The rather recent development of genetically encoded metabolite sensors has changed the way we can study metabolism in living cells, ex vivo tissues, and in vivo immensely. In recent years, these sensors have also been adapted for use in Drosophila tissues. Here, we describe a standard protocol to image such sensors in ex vivo Drosophila larval brains using the glucose sensor FLII12Pglu-700μδ6. The protocol, however, can be adapted for the use of other sensors, tissues, and can even be used in vivo.
Details
Original language | English |
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Title of host publication | Drosophila: Methods and Protocols |
Publisher | Springer |
Pages | 401-414 |
Number of pages | 14 |
Publication status | Published - 19 Aug 2022 |
Peer-reviewed | Yes |
External IDs
Scopus | 85136703438 |
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Keywords
ASJC Scopus subject areas
Keywords
- Carbohydrate transport, FRET, Genetically encoded metabolite sensors, Live imaging, Metabolism