Genetically Encoded Sensors to Study Metabolism in Drosophila

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportInvitedpeer-review



The rather recent development of genetically encoded metabolite sensors has changed the way we can study metabolism in living cells, ex vivo tissues, and in vivo immensely. In recent years, these sensors have also been adapted for use in Drosophila tissues. Here, we describe a standard protocol to image such sensors in ex vivo Drosophila larval brains using the glucose sensor FLII12Pglu-700μδ6. The protocol, however, can be adapted for the use of other sensors, tissues, and can even be used in vivo.


Original languageEnglish
Title of host publicationDrosophila: Methods and Protocols
Publication statusPublished - 19 Aug 2022

External IDs

Scopus 85136703438