The combination of the human induced pluripotent stem cell (hiPSC) and organoid technology enables the generation of human 3D culture systems, providing the opportunity to model human tissue-like structures in vitro. This protocol offers the details to generate and characterize self-assembling 3D cardiac organoids in a controlled and efficient manner based on hiPSC-derived cardiomyocytes. Cardiac organoids can be used as 3D-based assay systems and offer a wide range of applications in pharmacological and toxicological research as well as an alternative to animal experiments.
|Number of pages||16|
|Publication status||Published - Sept 2022|
ASJC Scopus subject areas
- Cardiac organoids, Cell culture, Disease modelling, Drug screening, Heart, iPSCs, Pharmacology, Stem cells, Toxicity screening