Fluorescently Labeled Cyclodextrin Derivatives as Exogenous Markers for Real-Time Transcutaneous Measurement of Renal Function

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Jiaguo Huang - , Heidelberg University  (Author)
  • Stefanie Weinfurter - , Heidelberg University  (Author)
  • Pedro Caetano Pinto - , Utrecht University (Author)
  • Marc Pretze - , Heidelberg University  (Author)
  • Bettina Kränzlin - , Heidelberg University  (Author)
  • Johannes Pill - , Heidelberg University  (Author)
  • Rodeghiero Federica - , Cyanagen S.r.l (Author)
  • Rossana Perciaccante - , Cyanagen S.r.l (Author)
  • Leopoldo Della Ciana - , Cyanagen S.r.l (Author)
  • Rosalinde Masereeuw - , Utrecht University (Author)
  • Norbert Gretz - , Heidelberg University  (Author)

Abstract

Evaluation of renal function is crucial for a number of clinical situations. Here, we reported a novel exogenous fluorescent marker (FITC-HPβCD) to real-time assess renal function by using a transcutaneous fluorescent detection technique. FITC-HPβCD was designed based on the principle of renal clearance of designed drugs. It displays favorable fluorescent properties, high hydrophilicity, low plasma protein binding, and high stability in porcine liver esterase as well as in plasma and nontoxicity. More importantly, FITC-HPβCD can be efficiently and rapidly filtered by glomerulus and completely excreted into urine without proximal tubular reabsorption or secretion in rat models. Additionally, the marker was well-tolerated, with nearly 100% urinary recovery of the given doses, and no metabolism were found. Relying on this novel kidney function marker and transcutaneous devices, we demonstrate a rapid, robust, and convenient approach for real-time assessing renal function without the need of time-consuming blood and urine sample preparation. Our work provides a promising tool for noninvasive real-time monitoring of renal function in vivo.

Details

Original languageEnglish
Pages (from-to)2513-2526
Number of pages14
JournalBioconjugate chemistry
Volume27
Issue number10
Publication statusPublished - 19 Oct 2016
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 84992313035
ORCID /0000-0002-6432-5694/work/146644242

Keywords

Keywords

  • Animals, Biomarkers/chemistry, Blood Proteins/metabolism, Cell Survival/drug effects, Chemistry Techniques, Synthetic, Cyclodextrins/chemistry, Drug Stability, Esterases/metabolism, Fluorescein/chemistry, Fluorescein-5-isothiocyanate/chemistry, Fluorescent Dyes/chemistry, Humans, Kidney Function Tests/instrumentation, Optics and Photonics/methods, Rats, Sprague-Dawley, Swine

Library keywords