Fluorescence correlation spectroscopy in membrane structure elucidation
Research output: Contribution to journal › Review article › Contributed › peer-review
Contributors
Abstract
This review describes the application of fluorescence correlation spectroscopy (FCS) for the study of biological membranes. Monitoring the fluorescence signal fluctuations, it is possible to obtain diffusion constants and concentrations for several membrane components. Focusing the attention on lipid bilayers, we explain the technical difficulties and the new FCS-based methodologies introduced to overcome them. Finally, we report several examples of studies which apply FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization.
Details
Original language | English |
---|---|
Pages (from-to) | 225-233 |
Number of pages | 9 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 1788 |
Issue number | 1 |
Publication status | Published - Jan 2009 |
Peer-reviewed | Yes |
External IDs
PubMed | 18801332 |
---|
Keywords
ASJC Scopus subject areas
Keywords
- Anomalous diffusion, FCS, Fence model, Membrane dynamics, Picket model, Raft