Oxford Nanopore Technologies allows sequencing of native RNA for the first time. Additionally they released tiny devices that democratize sequencing among scientists. However, the smallest sequencing device - Flongle - is so far not officially supported for RNA sequencing experiments.

This protocol provides users with a personally tested framework protocol to use Flongle flow-cells for native RNA sequencing. It is based on the protocol for ONT RNA library preparation and changed to fit the volume and input requirements for Flongle.
The here advised workflow will routinely result in 100,000 native RNA reads within 24 hours of sequencing.