Feline foamy virus genome and replication strategy
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus.
Details
Original language | English |
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Pages (from-to) | 11324-31 |
Number of pages | 8 |
Journal | Journal of virology |
Volume | 77 |
Issue number | 21 |
Publication status | Published - Nov 2003 |
Peer-reviewed | Yes |
External IDs
PubMedCentral | PMC229293 |
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Scopus | 0142124181 |
ORCID | /0000-0002-0320-4223/work/150884971 |
Keywords
Keywords
- Animals, Cats, Cell Line, Cricetinae, DNA, Viral/genetics, Genome, Viral, Humans, Mice, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Viral/genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Spumavirus/genetics, Transfection, Virion/metabolism, Virus Replication