Purpose: To compare quantitative cartilage volume measurement (CVM) using different slice thicknesses. Materials and methods: Ten knees were scanned with a 1.5 T MRI (Sonata, Siemens, Erlangen, Germany) using a 3Dgradient echo sequence (FLASH, fast low-angle shot). Cartilage volume of themedial and lateral tibial plateau was measured by two independent readers in 1.5mm, 3.0mm and 5.0mm slices using the Argus® software application. Accuracy and time effectiveness served as control parameters. Results: Determining cartilage volume, time for calculation diminished for the lateral tibial plateau from 384.6±127.7 s and 379.1±117.6 s to 214.9±109.9 s and 213.9±102.2 s to 122.1±60.1 s and 126.8±56.2 s and for themedial tibial plateau from465.0±147.7 s and 461.8±142.7 s to 214.0±67.9 s and 208.9±66.2 s to 132.6±41.5 s and 130.6±42.0 s measuring 1.5mm, 3mmand 5mmslices, respectively. No statistically significant difference between cartilage volume measurements was observed (p > 0.05) while very good inter-reader correlation was evaluated. Conclusion: CVM using 1.5mmslices provides no higher accuracy than cartilage volume measurement in 5mm slices while an overall time saving up to 70% is possible.