Encapsulated living cells on microstructured surfaces

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Silke Krol - , University of Genoa (Author)
  • Marc Nolte - , Max Planck Institute of Colloids and Interfaces (Author)
  • Alberto Diaspro - , University of Genoa (Author)
  • Davide Mazza - , University of Genoa (Author)
  • Raffaella Magrassi - , University of Genoa (Author)
  • Alessandra Gliozzi - , University of Genoa (Author)
  • Andreas Fery - , Max Planck Institute of Colloids and Interfaces (Author)

Abstract

The immobilization of cells in defined arrays (cell patterning) is a key step towards cell-based biosensors or other cell-based devices. While cell patterning is usually achieved by modifying the surface on which only the cells should adhere and leaving the cells unmodified, we present here a different approach in which cells are first coated with polyelectrolytes and subsequently immobilized on patterned surfaces. By coating, the cells are protected and their interactions with the substrate are modified such that patterning is simplified. We used microcontact printing of polyelectrolytes to structure surfaces such that regions of opposite charges and the same charge as the cell coating were present and found that we can thus achieve patterning of the coated yeast cells. In accordance with prior work, we find that coating does not kill the cells and coated GFP-expressing cells still function after immobilization, which we checked by fluorescence microscopy.

Details

Original languageEnglish
Pages (from-to)705-709
Number of pages5
JournalLangmuir
Volume21
Issue number2
Publication statusPublished - 18 Jan 2005
Peer-reviewedYes
Externally publishedYes

External IDs

PubMed 15641843