Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels

Research output: Contribution to journalResearch articleContributedpeer-review



Biopolymer hydrogels have become an important group of biomaterials in experimental and clinical use. However, unlike metallic or mineral materials, they are quite sensitive to sterilization. The aim of this study was to compare the effects of gamma irradiation and supercritical carbon dioxide (scCO 2) treatment on the physicochemical properties of different hyaluronan (HA)- and/or gelatin (GEL)-based hydrogels and the cellular response of human bone marrow-derived mesenchymal stem cells (hBMSC). Hydrogels were photo-polymerized from methacrylated HA, methacrylated GEL, or a mixture of GEL/HA. The composition and sterilization methods altered the dissolution behavior of the biopolymeric hydrogels. There were no significant differences in methacrylated GEL release but increased methacrylated HA degradation of gamma-irradiated samples. Pore size/form remained unchanged, while gamma irradiation decreased the elastic modulus from about 29 kPa to 19 kPa compared to aseptic samples. HBMSC proliferated and increased alkaline phosphatase activity (ALP) particularly in aseptic and gamma-irradiated methacrylated GEL/HA hydrogels alike, while scCO 2 treatment had a negative effect on both proliferation and osteogenic differentiation. Thus, gamma-irradiated methacrylated GEL/HA hydrogels are a promising base for multi-component bone substitute materials.


Original languageEnglish
Article number317
Number of pages16
JournalJournal of functional biomaterials
Issue number6
Publication statusPublished - 8 Jun 2023

External IDs

unpaywall 10.3390/jfb14060317
PubMed 37367281
WOS 001017417200001
Scopus 85163751205
ORCID /0000-0002-5611-9903/work/142244052
ORCID /0000-0003-2285-3621/work/142255134
Mendeley be03c184-4af7-3937-95cf-775feb209625


Research priority areas of TU Dresden

DFG Classification of Subject Areas according to Review Boards

ASJC Scopus subject areas


  • Human bone marrow stromal cells, Hydrogels, Methacrylated gelatin, Methacrylated hyaluronan, Osteogenic differentiation, methacrylated gelatin, methacrylated hyaluronan, osteogenic differentiation, human bone marrow stromal cells, hydrogels