Effect of chondroitin sulfate on osteogenetic differentiation of human mesenchymal stem cells

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Wolfgang Schneiders - , University Hospital Carl Gustav Carus Dresden, University Center for Orthopedics, Trauma and Plastic Surgery (OUPC) (Author)
  • Claudia Rentsch - , University Hospital Carl Gustav Carus Dresden, University Center for Orthopedics, Trauma and Plastic Surgery (OUPC) (Author)
  • Sebastian Rehberg - , University Hospital Carl Gustav Carus Dresden, University Center for Orthopedics, Trauma and Plastic Surgery (OUPC) (Author)
  • Susanne Rein - , University Hospital Carl Gustav Carus Dresden, University Center for Orthopedics, Trauma and Plastic Surgery (OUPC) (Author)
  • Hans Zwipp - , University Hospital Carl Gustav Carus Dresden, University Center for Orthopedics, Trauma and Plastic Surgery (OUPC) (Author)
  • Stefan Rammelt - , University Center for Orthopedics, Trauma and Plastic Surgery, University Hospital Carl Gustav Carus Dresden (Author)

Abstract

Chondroitin sulfate (CS) has anti-inflammatory properties and increases the regeneration ability of injured bone. In different in vivo investigations on bone defects the addition of CS to calcium phosphate bone cement has lead to an enhanced bone remodeling and increased new bone formation. The goal of this study was to evaluate the cellular effects of CS on human mesenchymal stem cells (hMSCs). In cell culture experiments hMSCs were incubated on calcium phosphate bone cements with and without CS and cultivated in a proliferation and an osteogenetic differentiation media. Alkaline phosphatase and the proliferation rate were determined on days 1, 7 and 14. Concerning the proliferation rates, no significant differences were detected. On days 1, 7 and 14 a significantly higher activity of alkaline phosphatase, an early marker of osteogenesis, was detected around CS modified cements in both types of media. The addition of CS leads to a significant increase of osteogenetic differentiation of hMSCs. To evaluate the influence of the osteoconductive potency of CS in twelve adult male Wistar rats, the interface reaction of cancellous bone to a nanocrystalline hydroxyapatite cement containing type I collagen (CDHA/Coll) without and with CS (CDHA/Coll/CS) was evaluated. Cylindrical implants were inserted press-fit into a defect of the tibial head. 28 days after the operation the direct bone contact and the percentage of newly formed bone were significantly higher on CDHA/Coll/CS-implants (p < 0.05). The addition of CS appears to enhance new bone formation on CDHA/Coll-composites in the early stages of bone healing. Possible mechanisms are discussed.

Details

Original languageEnglish
Pages (from-to)1926-1930
Number of pages5
JournalMaterials Science and Engineering C
Volume32
Issue number7
Publication statusPublished - 1 Oct 2012
Peer-reviewedYes

Keywords

Keywords

  • Calcium phosphate cement, Chondroitin sulfate, Human mesenchymal stem cells