E-Cadherin restricts mast cell degranulation in mice

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Crosslinking of FcεRI-bound IgE triggers the release of a large number of biologically active, potentially anaphylactic compounds by mast cells. FcεRI activation ought to be well-controlled to restrict adverse activation. As mast cells are embedded in tissues, adhesion molecules may contribute to limiting premature activation. Here, we report that E-Cadherin serves that purpose. Having confirmed that cultured mast cells express E-Cadherin, a mast-cell-specific E-Cadherin deficiency, Mcpt5-Cre E-Cdhfl/fl mice, was used to analyze mast cell degranulation in vitro and in vivo. Cultured peritoneal mast cells from Mcpt5-Cre E-Cdhfl/fl mice were normal with respect to many parameters but showed much-enhanced degranulation in three independent assays. Soluble E-Cadherin reduced the degranulation of control cells. The release of some newly synthesized inflammatory cytokines was decreased by E-Cadherin deficiency. Compared to controls, Mcpt5-Cre E-Cdhfl/fl mice reacted much stronger to IgE-dependent stimuli, developing anaphylactic shock. We suggest E-Cadherin-mediated tissue interactions restrict mast cell degranulation to prevent their precocious activation.

Details

Original languageEnglish
Pages (from-to)44-53
Number of pages10
JournalEuropean Journal of Immunology
Volume52
Issue number1
Publication statusPublished - Jan 2022
Peer-reviewedYes

External IDs

Scopus 85117473486

Keywords

Keywords

  • Animals, Cadherins/genetics, Cell Degranulation/genetics, Cytokines/genetics, Immunoglobulin E/genetics, Inflammation/genetics, Mast Cells/immunology, Mice, Mice, Transgenic, Receptors, IgE/genetics