Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Nahla Ibrahim - , University Hospital Vienna (Author)
  • Johannes Klopf - , University Hospital Vienna (Author)
  • Sonja Bleichert - , University Hospital Vienna (Author)
  • Marc A Bailey - , University of Leeds (Author)
  • Albert Busch - , Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus Dresden (Author)
  • Alexander Stiglbauer-Tscholakoff - , University Hospital Vienna (Author)
  • Wolf Eilenberg - , University Hospital Vienna (Author)
  • Christoph Neumayer - , University Hospital Vienna (Author)
  • Christine Brostjan - , University Hospital Vienna (Author)

Abstract

Since pharmaceutical treatment options are lacking in the clinical management of abdominal aortic aneurysm (AAA), animal models, in particular mouse models, are applied to advance the understanding of the disease pathogenesis and to identify potential therapeutic targets. Testing novel drug candidates to block AAA growth in these models generally requires repeated drug administration during the time course of the experiment. Here, we describe a compiled protocol for AAA induction, insertion of an intravenous catheter to facilitate prolonged therapy, and serial AAA monitoring by 3D ultrasound. Aneurysms are induced in apolipoprotein E (ApoE) deficient mice by angiotensin II release over 28 days from osmotic mini-pumps implanted subcutaneously into the mouse back. Subsequently, the surgical procedure for external jugular vein catheterization is conducted to allow for daily intravenous drug treatment or repeated blood sampling via a subcutaneous vascular access button. Despite the two dorsal implants, the monitoring of AAA development is readily facilitated by sequential semi-automated 3D ultrasound analysis, which yields comprehensive information on the expansion of aortic diameter and volume and on aneurysm morphology, as illustrated by experimental examples.

Details

Original languageEnglish
Article numbere64124
JournalJournal of visualized experiments : JoVE
Volume2022
Issue number186
Publication statusPublished - 4 Aug 2022
Peer-reviewedYes

External IDs

Scopus 85136181727

Keywords

Keywords

  • Angiotensin II/adverse effects, Animals, Aorta, Abdominal/diagnostic imaging, Aortic Aneurysm, Abdominal/chemically induced, Apolipoproteins E, Catheterization, Central Venous Catheters, Disease Models, Animal, Mice, Mice, Inbred C57BL, Mice, Knockout, Ultrasonography

Library keywords