Optical coherence tomography (OCT) in combination with Doppler analysis (DOCT) is used to image the vibration response of the human tympanic membrane (TM). Free-field sound excitation was applied repeatedly in an acoustic frequency range of 0.4 to 5.1 kHz. In this study, two ex vivo TM samples were used - one non-pathological and one thickened prior around the manubrium of malleus. In spite of the minor oscillation amplitude of the pathological TM, comparable vibration modes can be identified even though occurring at lower acoustic excitation frequencies.