Developing Cole-Cole model for Bacillus subtilis fermentation

Research output: Contribution to book/Conference proceedings/Anthology/ReportChapter in book/Anthology/ReportContributedpeer-review

Contributors

Abstract

During the fermentation of Bacillus Subtilis it is unperceivable to observe the formation of spores since, following the evolution of spores is time consuming due to various sampling and analytical procedures. Dielectric spectroscopy has shown promising results, where the measured permittivity is correlated with the cell concentration. Most of the existing works focus on determining cell concentration and not on the determination of spores. In this work, we develop a permittivity model for cells in the fermentation solution based on the Cole-Cole equations that can be used to detect the sporulation phase in future work. Utilizing the data from several fermentation runs of Bacillus Subtilis, a superposition hypothesis is proposed. In this hypothesis, the model equations from Cole and Cole (1941) are used and the theoretical permittivity response of the cells is calculated. As a first step, the Cole-Cole model is developed, and the modeled permittivity values are fitted to the measured data and the results are analyzed.

Details

Original languageEnglish
Title of host publicationProceedings 33rd European Symposium on Computer Aided Process Engineering
PublisherElsevier B.V. on behalf of KeAi Communications Co. Ltd.
Pages2711-2716
Number of pages6
Publication statusPublished - Jan 2023
Peer-reviewedYes

Publication series

Series Computer aided chemical engineering
Volume52
ISSN1570-7946

External IDs

ORCID /0000-0001-5165-4459/work/174432574

Keywords

Research priority areas of TU Dresden

DFG Classification of Subject Areas according to Review Boards

Subject groups, research areas, subject areas according to Destatis

Keywords

  • Cole-Cole, Permittivity, Sporulation, Superposition