Dendritic cell podosome dynamics does not depend on the F-actin regulator SWAP-70
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
In addition to classical adhesion structures like filopodia or focal adhesions, dendritic cells similar to macrophages and osteoclasts assemble highly dynamic F-actin structures called podosomes. They are involved in cellular processes such as extracellular matrix degradation, bone resorption by osteoclasts, and trans-cellular diapedesis of lymphocytes. Besides adhesion and migration, podosomes enable dendritic cells to degrade connective tissue by matrix metalloproteinases. SWAP-70 interacts with RhoGTPases and F-actin and regulates migration of dendritic cells. SWAP-70 deficient osteoclasts are impaired in F-actin-ring formation and bone resorption. In the present study, we demonstrate that SWAP-70 is not required for podosome formation and F-actin turnover in dendritic cells. Furthermore, we found that toll-like receptor 4 ligand induced podosome disassembly and podosome-mediated matrix degradation is not affected by SWAP-70 in dendritic cells. Thus, podosome formation and function in dendritic cells is independent of SWAP-70.
Details
Original language | English |
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Pages (from-to) | e60642 |
Journal | PLoS ONE |
Volume | 8 |
Issue number | 3 |
Publication status | Published - 2013 |
Peer-reviewed | Yes |
External IDs
PubMed | 23544157 |
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PubMedCentral | PMC3609734 |
Scopus | 84875460283 |
Keywords
Keywords
- Actins/metabolism, Animals, Cell Membrane Structures/metabolism, DNA-Binding Proteins/deficiency, Dendritic Cells/cytology, Extracellular Space/metabolism, Gelatin/metabolism, Guanine Nucleotide Exchange Factors/deficiency, Humans, Lipopolysaccharides/pharmacology, Mice, Minor Histocompatibility Antigens, Nuclear Proteins/deficiency, Protein Transport, Toll-Like Receptor 4/metabolism, Toll-Like Receptors/metabolism