Direct laser interference patterning (DLIP) employing sub-picosecond IR irradiation allows for the ultrafast processing of polyaniline surfaces polymerized onto a poly(methyl methacrylate) substrate (PANI@PMMA) achieving excellent quality features. The patterning shows high levels of precision regarding control of width and height in micro/nanoarrays, giving customization to the surfaces. The electroactive property evaluation suggests the conductivity presents anisotropic characteristics following the patterning of the polymeric surfaces (R _SL= 54 ± 5 kω sq ^-1and R _ST= 1.7 ± 3 Mω sq ^-1, longitudinal and transversal resistance modes respectively). The evidence is supported by electrostatic force microscopy measurements. These results indicate potential applicability in the biomedical field of nerve and myocardial tissue regeneration.