Consensus statement on Brillouin light scattering microscopy of biological materials

Research output: Contribution to journalReview articleContributedpeer-review

Contributors

  • Pierre Bouvet - , Medical University of Vienna (Author)
  • Carlo Bevilacqua - , European Molecular Biology Laboratory (EMBL) Heidelberg (Author)
  • Yogeshwari Ambekar - , University of Maryland, College Park (Author)
  • Giuseppe Antonacci - , Specto S.r.l. (Author)
  • Joshua Au - , University of Maryland, College Park (Author)
  • Silvia Caponi - , National Research Council of Italy (CNR) (Author)
  • Sophie Chagnon-Lessard - , LightMachinery Inc., Carleton University (Author)
  • Juergen Czarske - , Chair of Measurement and Sensor System Technique, Clusters of Excellence PoL: Physics of Life (Author)
  • Thomas Dehoux - , Universite Claude Bernard Lyon 1 (Author)
  • Daniele Fioretto - , University of Perugia (Author)
  • Yujian Fu - , Medical University of Vienna (Author)
  • Jochen Guck - , Max Planck Institute for the Science of Light (Author)
  • Thorsten Hamann - , Norwegian University of Science and Technology (Author)
  • Dag Heinemann - , Leibniz University Hannover (LUH) (Author)
  • Torsten Jähnke - , CellSense Technologies GmbH (Author)
  • Hubert Jean-Ruel - , Carleton University (Author)
  • Irina Kabakova - , University of Technology Sydney (Author)
  • Kristie Koski - , University of California at Davis (Author)
  • Nektarios Koukourakis - , Chair of Measurement and Sensor System Technique, Clusters of Excellence PoL: Physics of Life (Author)
  • David Krause - , Chair of Measurement and Sensor System Technique, Clusters of Excellence PoL: Physics of Life (Author)
  • Salvatore La Cavera - , University of Nottingham (Author)
  • Timm Landes - , Leibniz University Hannover (LUH) (Author)
  • Jinhao Li - , European Molecular Biology Laboratory (EMBL) Heidelberg (Author)
  • Hadi Mahmodi - , University of Technology Sydney (Author)
  • Jeremie Margueritat - , Universite Claude Bernard Lyon 1 (Author)
  • Maurizio Mattarelli - , University of Perugia (Author)
  • Michael Monaghan - , Trinity College Dublin (Author)
  • Darryl R. Overby - , Imperial College London (Author)
  • Fernando Perez-Cota - , University of Nottingham (Author)
  • Emanuele Pontecorvo - , CrestOptics S.p.A. (Author)
  • Robert Prevedel - , European Molecular Biology Laboratory (EMBL) Heidelberg, Translational Lung Research Center Heidelberg (TLRC) - DZL Heidelberg (Author)
  • Giancarlo Ruocco - , Italian Institute of Technology (Author)
  • John Sandercock - , The Table Stable Ltd (Author)
  • Giuliano Scarcelli - , University of Maryland, College Park (Author)
  • Filippo Scarponi - , The Table Stable Ltd (Author)
  • Claudia Testi - , Italian Institute of Technology (Author)
  • Peter Török - , Nanyang Technological University (Author)
  • Lucie Vovard - , Universite Claude Bernard Lyon 1 (Author)
  • Wolfgang J. Weninger - , Medical University of Vienna (Author)
  • Vladislav Yakovlev - , Texas A&M University (Author)
  • Seok Hyun Yun - , Harvard University (Author)
  • Jitao Zhang - , Wayne State University, Michigan State University (Author)
  • Francesca Palombo - , University of Exeter (Author)
  • Alberto Bilenca - , Ben-Gurion University of the Negev (Author)
  • Kareem Elsayad - , Medical University of Vienna (Author)

Abstract

Brillouin light scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the submicrometre scale. Over the past decade, BLS has found increasing microscopy applications in the life sciences, driven by the observed importance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and the extension of BLS to an imaging modality. As with other spectroscopic techniques, BLS measurements detect not only signals that are characteristic of the investigated sample, but also those of the experimental apparatus, and can be substantially affected by measurement conditions. Here we report a consensus between researchers in the field. We aim to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artefacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different, often self-built, spectrometers, a consensus statement is particularly timely to ensure unified advancement.

Details

Original languageEnglish
Pages (from-to)681-691
Number of pages11
JournalNature Photonics
Volume19
Issue number7
Publication statusPublished - 3 Jul 2025
Peer-reviewedYes

Keywords

Keywords

  • Brillouin confocal microscopy